| Previous day | Friday 3 Jun 2016 | Next day |
| Sam Houston State 3 vs Arizona 7 | Lafayette Regional Game 1 | |
| Gonzaga 5 vs Arizona State 1 | Fort Worth Regional Game 1 | |
| East Carolina 9 vs Bryant 1 | Charlottesville Regional Game 2 | |
| Louisiana Tech vs Cal State Fullerton | "Starkville Regional Game 2 (Suspended to 6/4; 0-0, b7)" | |
| Western Carolina 10 vs Clemson 24 | Clemson Regional Game 2 | |
| St Mary's 2 vs Coastal Carolina 5 | Raleigh Regional Game 1 | |
| New Mexico 12 vs Dallas Baptist 6 | Lubbock Regional Game 2 | |
| Bethune-Cookman 3 vs Florida 9 | Gainesville Regional Game 2 | |
| Long Beach State 5 vs Florida Atlantic 1 | Coral Gables Regional Game 1 | |
| Alabama State 6 vs Florida State 18 | Tallahassee Regional Game 2 | |
| Connecticut 7 vs Georgia Tech 6 | Gainesville Regional Game 1 | |
| Utah Valley State 1 vs Louisiana State 7 | Baton Rouge Regional Game 1 | |
| Princeton 3 vs Louisiana-Lafayette 5 | Lafayette Regional Game 2 | |
| Western Michigan 1 vs Louisville 6 | Louisville Regional Game 2 | |
| Stetson 2 vs Miami Florida 4 | Coral Gables Regional Game 2 | |
| Wake Forest 5 vs Minnesota 3 | College Station Regional Game 1 | |
| Utah 6 vs Mississippi 5 (10) | Oxford Regional Game 2 | |
| Southeast Missouri State 5 vs Mississippi State 9 | Starkville Regional Game 1 | |
| Navy 8 vs North Carolina State 13 | Raleigh Regional Game 2 | |
| Duke 1 vs North Carolina-Wilmington 11 | Columbia Regional Game 1 | |
| Wright State 6 vs Ohio State 7 | Louisville Regional Game 1 | |
| Nebraska 0 vs Oklahoma State 6 | Clemson Regional Game 1 | |
| Southeastern Louisiana vs Rice | "Baton Rouge Regional Game 2 (PPD to 6/4, rain)" | |
| Rhode Island 5 vs South Carolina 4 | Columbia Regional Game 2 | |
| South Alabama 2 vs Southern Mississippi 14 | Tallahassee Regional Game 1 | |
| Binghamton 2 vs Texas A&M 4 | College Station Regional Game 2 | |
| Oral Roberts 0 vs Texas Christian 7 | Fort Worth Regional Game 2 | |
| Fairfield 1 vs Texas Tech 12 | Lubbock Regional Game 1 | |
| Boston College 7 vs Tulane 2 | Oxford Regional Game 1 | |
| Washington 2 vs UC Santa Barbara 3 (14) | Nashville Regional Game 1 | |
| Xavier vs Vanderbilt | "Nashville Regional Game 2 (PPD to 6/4, weather)" | |
| William and Mary 4 vs Virginia 17 | Charlottesville Regional Game 1 |